Is cytochrome c oxidase subunit I (COI) the right DNA barcoding marker for the Chaetopteryx villosa group?

Chaetopteryx villosa (Fabricius, 1798) is a caddisfly species distributed throughout Europe, except in the Balkan and Apennine Peninsula. However, phylogenetically close belonging to C. group are widespread entire Europe. Species of this ( , gessneri fusca sahlbergi atlantica, bosniaca vulture, trinacriae ) have distinct distributions with some overlaps. Adult forms these morphologically similar, whereas larval morphology only known for species. There also indications hybridization (e.g., x ). Presumably, molecular approach determination would be highly beneficial. In BOLD database, there 154 specimens COI-5P barcodes Out remaining species, has 27 barcode, 20, 5, atlantica 1, sequences from vulture missing. Therefore, we tested power discrimination COI -5P marker group, as most common barcoding markers identification animals. Only public records originating experienced research groups or taxonomists containing specimen photograph were taken input. A total 75 database obtained. sequences, 11 belonged 5 52 bosniaca, 2 . For generation overview trees, Rhyacophila fasciata Rh. nubila used outgroups. All trimmed at 5’ 3’ ends, resulting final alignment length 516 base pairs. Multiple sequence alignments editing done MEGA-X software. Analysis nucleotide polymorphism was DNASP6 calculate pairwise distance overall mean p-distance, construct trees. DNA revealed 14 haplotypes 3 gessneri, one no significant interspecific intraspecific differences among based on distances. The p-distance between 0.000, varied 0.001 about 0.055. all equaled 0.03. No species-specific clusters observed when phylogenetic trees constructed regardless method (i.e., NJ, UPGMA, ML, ME, MP). To minimize possibility misidentification, submitted by NTNU-Norwegian University Science Technology (Norway), SNSB-Zoologische Staatssammlung Muenchen (Germany), Zoologisches Forschungsmuseum Alexander Koenig Oulu, Zoological Museum (Finland), prof Hans Malicky Mladen Kučinić. identified hybrids included analyses. With exception failed separate group. it unlikely that poor basis such result. enable comprehensive unbiased evaluation relationships within data coverage studied should enhanced, encompassing different geographical distribution samples. Further studies needed detect array suitable delineation complex